Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Antibacterial Activity Of Prenylated Xanthones From Pericarp Of Garcinia Mangostana Against Persistent Dental Infection Microorganism Enterococcus Faecalis

As a part of ongoing research on antibacterial agents from botanical dietary supplements, Garcinia mangostana L. (commonly known as manggis in Indonesia) was selected for a detailed study. The dried and milled pericarp of G. mangostana was extracted by maceration with MeOH (3 × 5 L) at room temperature for 3 days. After filtration and evaporation of the solvent under reduced pressure, the combined crude methanolic extract (250 g) was suspended in H2O (600mL) to produce an aqueous solution, then partitioned in turn with n-hexane (3 × 500 mL), EtOAc (3 × 500 mL) and n-BuOH (3 × 500 mL) to afford dried nhexane (32 g), EtOAc (40 g) and n-BuOH (60 g) extracts. The EtOAc-soluble extract was found to have a significant antibacterial activity against Enterococcus faecalis ATCC 29212. Therefore, the EtOAc extract was selected for detailed purification. Repeated chromatography on silica gel of a EtOAc-soluble extract of pericarp led to isolation of four prenylated xanthones. The chemical structures of compounds 1-4 were identified as a-mangostin, b-mangostin, gmangostin and garcinone-D on the basis of spectroscopic data and comparison to those related data previously reported. The antibacterial activities of these compounds were evaluated against E. faecalis performed using broth micro dilution method. Among all compounds, compound 3 (g-mangostin) exhibit the most potent since it has a high inhibition diameter value in low concentration (10.93 d/mm in 100 mg/L).