Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Application Of Spe-gc/ms For Analysis Lead Compounds Aromatherapy In Blood Plasma Of Mice Of Essential Oils Materials From Insonesian Spice

Leads compunds is one of fulfilled in standardization of Indonesian drug medicine standardized, but essential oils of material of aromatherapy standardized don’t find of essential oils material from Indonesia. The result showed that the four essential oils gave an inhibitory effect on locomotor activity of the mice. Inhalation of essential oils of kemangi leaves, serai dapur herbs, pala seeds and ki lemo bark decreased locomotor with the percent inhibition up to 57,64 %, 55,72 %, 68,62 %, and 60,75 %, respectively. Identification and quantification of active compounds in the blood plasma were carried out with GC/MS analysis after the mice experienced half an hour, one hour, and two hours inhalation. The blood plasma of three mice were collected in heparin tube and the volatile compounds were isolated and concentrated by the C-18 column (100 mg-Sep-Pak) with methanol and bidistilled water mixture (60:40) as the eluent. The recovery analysis was increased up to 90 %. Major volatile compounds identified from blood plasma of the mice after inhalation of the essential oil of kemangi leaves were linalool and linaly acetate, whereas myristicin, 4-terpineol, and esthers of chain length (methyl palmitate, methyl myristate, methyl oleate, and methyl stearate) were dominant in blood plasma of mice after inhlation of the the essential oil of nutmeg seeds, and citronellol and citronellal dominant only in the blood plasma of mice inhaled essential oil of ki lemo barks. Linalool,citronelal, citral, dan methyl cinnamate are cimpounds identified from blood plasma of the mice after inhalation of the essential oil of serai dapur herbs.