Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Plant Regeneration Capacity Of Calluses Derived From Mature Seed Of Five Indonesian Rice Genotypes

Establishment of a suitable system for plant regeneration of rice calluses derived from mature seed is a prerequisite for genetic transformation using callus as the target tissue. Selecting the most suitable medium and assessing the genotype performance for in vitro response are essential requirement for this purpose. The experiment with fi ve Indonesian rice genotypes showed that callus-proliferation capacity (CPC) and callus-growth capacity were signifi cantly affected by genotype and CPC by medium. The shoot-regeneration capacity and plantlet-regeneration capacity were affected by the interaction effect between genotype and medium. However for green plant-regeneration capacity, it was affected independently by genotype and medium. Culture media D1 and NB5 were the most suitable media for callus subculture and plant regeneration, respectively. Genotypes Fatmawati and BP-140 consistently performed best in the callus subculture and plant regeneration.