Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Isolation And Cloning Of Genes From Tuberizing And Non-tuberizing In Vitro Potato Explants

During in vitro tuberization, two contradictive phenomena have been found, potato explants could produce micro-tuber and another one could not generate micro-tuber. To elucidate such observable fact, isolation and cloning of important genes are effort should be undertaken. The aims of this research were to isolate and to clone genes, which differentially expressed between in vitro tuberizing and non-tuberizing potato explants. cDNA-AFLP (complementary DNA-amplified fragment length polymorphisms) technique was employed for such purpose. The cDNA-AFLP fragments labeled with ^733P-ATP were excised from the polyacrylamide gel.

Eight TDFs were isolated while only seven TDFs were successfully cloned into p-GEMT easy vector; three of these TDFs were isolated from non-tuberizing samples and the other, four bands were cut off from tuberizing samples. Seven plasmids containing DNA insert have been successfully introduced into Escherici~ co/i. Analysis of transformant bacteria, recombinant plasmid-DNA followed by digestion analysis using EccRI showed that some of plasmid vector contained TDFs or DNA insert of our interest.