Pustaka Ilmiah Universitas Padjadjaran

Abstrak

DNA-fingerprinting of downy mildew resistance (DMR) and quality protein maize (QPM) lines using eleven simple sequence repeats (SSR)

Maize cultivars possessing resistance to downy mildew pathogen and high quality protein is the ultimate goal of maize breeding program in Indonesia. A collaborative research between Lab. of Plant Breeding, Padjadjaran University (UNPAD) and Indonesia Com Research institute (ICERI) has been established to develop maize having these important traits. A set of experiment was conducted at the Field Experimental Stabon, Faculty of Agrkulture, Padjadjaran University in latinan^Dor and Lab. of Molecular Biology, Biotechnology Research Institute for Food Crop. Bogor to finger-print downy mildew resistance and quality protein of maize lines to be used as parental lines in the breeding program. Five of downy mildew resistant (DMR) inbred lines and six of quality protein maize (QPM) inbred lines were used in the study. Molecular characterization using eleven simple sequences repeats (SSR) was done to profile these inbred lines. There were three groups of parental lines based on Jackard coefficient similarity at 0.3.