Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Antagonistic Potency of Bacteria Isolated from Local Microorganism of Banana Stump in Suppressing Blast Disease (Pyricularia grisea [Cooke] Sacc) on Rice

Blast disease caused by Pyricularia grisea [Cooke] Sacc. is the most important disease on dry land rice. The use of antagonistic bacteria as bio-control agents is an alternative control method and friendly to environment.

The objective of the research was to isolate bacteria from Local Microorganism (MOL) of banana stump which were antagonistic to P. grisea in vitro and able to suppress blast on dry land rice var Cirata in glasshouse. The research was carried out in the Phytopathology Laboratory and Glasshouse of the Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran from December 2008 to March 2009.

There were two experiments i.e.: dual culture test on potato dextrose agar (PDA) and antagonism test on dry land rice var Cirata in the glasshouse. Both treatments were arranged in the completely randomized design consisted of 4 replications for in vitro and 3 replications for glasshouse test.

Among the fourteen bacterial isolates tested by dual culture, 7 isolates were antagonistic against P. grisea in vitro. The highest suppression was shown by B11BP isolate, the in vitro inhibition was 69,4%. However, the seven bacterial isolates were not effective in suppressing blast on dry land rice var Cirata in the glasshouse.