Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Determination and Genetic Variation Analysis of Wild-type and Albino’s Mutant of Monascus spp. by Random Amplification of Polymorphic DNA (RAPD) Method

Determination and genetic variation analysis of wild-type and albino’s mutan of Monascus spp. isolated from Cikapundung River at Bandung area, were carried out using Random Amplification of Polymorphic DNA (RAPD) method. The albino’s mutant, was obtained by Ethyl Methane Sulphonate (EMS)-treated spores of the wild-type. RAPD result of Monascus spp wild-type was compared with Monascus purpureus CECT 2955T as reference standard, and it was found that both RAPD profiles observed was identical. These result showed that the wild-type is proved to be Monascus purpureus. However, one of the DNA bands of albino’s mutant at 1150 bp was not found on the wild-type and the standard, and it was considered as a genetic variation resulted from the mutation process.