Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Characterization Of Methicillin Resistant Staphylococcus Aureus (Mrsa) Isolated From Human And Diary Cows

Staphylococcus aureus is the causative agent of clinical or subclinical mastitis in dairy cows. Staphylococcus aureus cause also pathogenic diseases in human. Several hospitals reported the increase of methicillin resistant S. aureus (MRSA) frequency, because this srtain was resistant to several antibiotics. The aim of research was to characterize S. aureus, distribution of resistant factor to methicillin and several other antibiotics.

The isolates of S.aureus originated from dairy cows (11 isolates) and from patients of Sarjito hospital (10 isolates). Bacterial identification based on the growth in PAD media, Gram staining, mannitol salt agar (MS) fermentation, catalase, coagulase, clumping factor tests and the resistancy/sensitivity to methicillin, penicillin G, ampicillin, oxacillin, tetracyclin, gentamycin and erythromycin. It was also identified to 23S rRNA and mecA genes for the resistancy screening to methicillin (MRSA) by using polymerise chain reaction (PCR).

All isolates from milk and human used in this study were S.aureus based on phenotypic and genotypic identifications. Staphylococcus aureus were resistant to penicillin group (20-100%), and to tetracyclin, gentamycin and erythromicin (9-40%). By PCR amplification could be observed for 90% methicillin resistant (mecA) genes for human isoates and 45% for milk isolates. The resistancy of S.aureus to methicillin in the present study might help to understand the distribution of methicillin resistant S. aureus (MRSA) among human and bovine isolates and might help to control S. aureus infecions in Indonesia.