Proceeding Biotech 
Antimicrobial Metabolite From The Culture Of Endophytic Fungus Afk-8 Isolated From Kayu Kuning [Archangelisia Flava (L.) Merr.]
Praptiwi, Yuliasri Jamal, Ahmad Fathoni Dan Andria Agusta
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Inggris
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antimicrobial activity, Arcangelisia flava, endophytic fungi, metabolites
The fungus AFK-8 is one of endophytic filamentous fungus isolated from young stems of kayu kuning [Archangelesia flava (L.) Merr.]. The ethyl acetate extract derived from fungus culture shows their antibiotic activity tested against bacteria and fungi on a disc diffusion method. Scaling up cultivation of endophytic fungus AFK-8 on 1L PDA for 3 weeks yielded 143.3 mg of chloroform extract. Fractionation guided assay of these extract showed that the fraction 6 is the most active fraction against tested microbes with MIC values 8, 32, 4 and 64 ??g/ml against B. subtilis, M. luteus, S. aureus dan E. coli respectively. This fraction is also active against C. albicans, R. minuta and A. niger with MIC value 32 mg/ml. The proposed chemical structure of active metabolit in fraction 6 is 1,2-diamino-9,10-anthracenedione which was determined through a GCMS analysis.