Proceeding Biotech 
Biooxidation Of Berberine By The Endophytic Fungus Coelomycetes Afkr-1 Isolated From Kayu Kuning [Archangelisia Flava (L.) Merr.: Menispermaceae]
Andria Agusta, Yuliasri Jamal, Praptiwi And Ahmad Fathoni
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Inggris
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Archangelisia flava, berberine, biotransformation, Coelomycetes AFKR-1, endophytic fungi
Totaly eighteen kinds of endophytic fungi have been isolated from the young stems of kayu kuning [Archangelisia flava (L.) Merr.] collected from Bogor Botanic Garden. All of isolated fungi have been screened for their capability to transform the host plant alkaloid berberine in two kinds of liquid mediums, potato dextrose broth (PDB) and glucose-yeast extract-peptone (GYP) respectively. Biotransformation reactions were monitored and analyzed by a normal phase thin layer chromatography technique. The screening process revealed that the fungus Coelomycetes AFKR-1 could be able to transform berberine into a biotransformed product in PDB medium. Scalingup biotransformation reactions (5 x 200 ml medium in 500 ml size Erlenmeyer) which were incubated at room temperature with agitation at 120 rpm yielded 20 % conversion rate of substrate into a product in 48 hrs. The chemical structure of biotransformed product was proposed as a 7-N-oxide-berberine based on their spectroscopic data.