Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Nucleotide Sequence For Primer Design Ssr Markers Of Mangosteen (Garcinia Mangostana L.)

Assessment and utilization of diversity in plant genetic resources is very important to improve of plant species. A mangosteen (Garcinia mangostana L.) seed mangosteen was formed obligate apomicts process. Genetic diversity of mangosteen was developed by using SSR markers. An inter-simple sequence repeat (ISSR)- supression-PCR technique established to SSR marker of plant spesies. DNA library construction was used restriction enzyme blunt end Rsa I and adaptor (consist of 48- mer: ‘-G5TAATACGACTCACTATAGGG CACGCGT GGTCGACGGCCCGGGCTGGT-3′ and 8-mer with the 3′-end capped by an amino residue: 5′-ACCAGCCC-NH2-3’). Primer designed by using compound SSR (AC)10; (TC)6(AC)5 or (AC)6(AG)5 and an adaptor primer AP2 and nested PCR using AP1 primers. The PCR product integrated into the plasmid PGEMT Easy Vector System and competence cell Escherichia coli (strain DH5a) and sequence. Eight sequence from sample #G17 with SSR compound (AC)10, (TC)6(AG)5 and (AC)6(AG)5 produced two primer pairs. An inter-simple sequence repeat (ISSR) suppression-PCR technique established to develop SSR markers of G. mangostana L. This method will be contributed greatly to analyze genetic diversity of G. mangostana L. or other species Garcinia.