Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Production Of 1,3-propanediol By Clostridium Butyricum P50b1 Using Commercial And Raw Glycerol From Biodiesel Production

Production of biodiesel (fatty acid methyl esters) by transesterification of palm oil generates glycerol as the main by-product. Different strains of Clostridium butyricum are able to produce 1,3-propanediol (1,3-PD) from glycerol. A new strain of C. butyricum was obtained during a screening for natural producers of 1,3-propanediol, and designated C. butyricum P50B1. Growth inhibition of C. butyricum P50B1 by commercial glycerol and raw glycerol from a palm oil-based biodiesel production process was evaluated. The strain exhibited the same tolerance to raw glycerol (87% w/v) and to commercial glycerol (87% w/v). Furthermore, 1,3-propanediol production from commercial and raw glycerol, without any prior purification, was observed in batch and fed-batch cultures on a synthetic medium. No significant differences were found in C. butyricum P50B1 fermentation patterns on raw and commercial glycerol as the sole carbon source. For both types of cultures, the conversion yield obtained was around 0.6 mol of 1,3-propanediol formed per 1 mol glycerol consumed. The highest end-concentration of 1,3-propanediol achieved during fed-batch cultures was around 57 g· l?1 , with productivity of 0.95 g·l?1 · h?1.