Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Selection Of Mx Gene Genotype As Genetic Marker For Avian Influenza Resistance In Indonesian Native Chicken

This research was aimed to select one of the native chicken breeds which found out resistance to avian influenza using the molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia. Marker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx– that corresponded to the positive antiviral activity (Mx++), or those which have could positive or negative activity (Mx+-) and negatively antiviral activity (Mx–). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that has Mx++ and Mx+- genotypes, were selected to produce offspring. Result showed that the resistant allele (Mx+) for laying hen had frequency of 65% and for cocks had frequency of 60%, while the susceptible allele (Mx-) for hen had frequency of 35% and for cocks had frequency of 40%. Age, egg weight and hen weight at first laying and egg production for susceptible genotype was slightly better than those the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 % respectively.