Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

The Presence Of Inclusion Bodies Of Helicoverpaarmigera Nuclear Polyhedrosis Virus (Hanpv) In The Midgut Tissue Of Fourth Instars Spodopteralitura Larvae

The research to determine the presence of inclusion bodies of HaNPV in the midgut tissue of fourth instars Spodopteraliturae larvae has been done. The larval has been infected by 1.13 X 105polihedra/ml concentration of viruses and the presence of inclusion bodies has been conducted every 8 hours from the 1st until 72nd hours after virus infection. The histological slide of midgut tissue of larvae has been stained by Mallory-Azan staining method. The experiment shows that in 8th hours after infection, the inclusion bodies of virus presence in columnar cells of larvae. In 24th hours after infection, inclusion bodies of virus through disgestive tract and appeared in faeces of larvae. In 32nd hours after infection, the inclusion boddies of virus still appeared in regenerative columnar cells until 56th hours after infection. After 64th hours until 72nd hours after infection, the present of polihedra damaged of midgut tissue, made all columnar cells lysis and inclusion bodies form into granul (polyhedral granules). Therefore HaNPV can replicated in Spodopteraliturae larval as an alternative host.