Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Dephytinisation Of Corn (Zea Mays L) By Citric Acid And Phytase’s Bacillus Subtilis Holiwood Gresik To Increase The Iron Dialyzability

The research objective was to increase the iron dialyzability in corn defitinisation through variation of soaking, addition of citric acid and phytase. The stages of the research: 1) isolation of crude phytase extracts from B.subtilis Holiwood Gresik isolate by One shot case study design, 2) determining the variation of soaking time and concentration of citric acid that produced the lowest phytate by Factorial design, 3) soaking in citric acid and addition of phytase to increase the iron dialyzability by Post test control group design. Data were analyzed by two-way and one-way Anova (a = 5%). Results: 1) The crude phytase extracts activity: 0.254 U/ml. 2) 12-hour soaking in 9% citric acid yielded the decreasing phytate content of corn to the remaining 31.789% and if accompanied by the addition of 250 µl/50 ml phytase yielded the decreasing until the remaining 6.559% of the original. 3) The use of citric acid alone and the combination of citric acid and phytase increased the iron dialyzability of corn by 3.8 -and 5.7-fold respectively.