Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Molecular Study Of Isolates Edwardsiella Tarda From Several Islands In Indonesia

Edwardsiella tarda is an enteric pathogen that causes diarrhea, wound infection, and death due to septicemia. This species is capable of invading fish and human epithelial cell lines, and E. tarda escapes from the endocytic vacuole within minutes of entry and then replicates within the cytoplasm moving directly from cell to cell. Edwardsiella tarda was much more frequently isolated from lake fish. The aim of research was to find out the strain variation of several isolates of E. tarda from different islands in Indonesia. Four isolates of E.tarda from catfish (Java and Sumatra), Cyprinus carpio (Kalimantan), Brazilian turtle (imported), and ATCC from Singapura were identified by morphology. All isolates were DNA extracted and DNA amplified by PCR. Products of amplification were digested with AluI and HaeIII enzymes (RFLP) for 1 hour. They were visualized with electrophoresis in 8% polyacrylamid gel and stained with silver. The restriction endonuclease result showed that 3 isolates from fish had the same bands. Two isolates from imported turtle had the same band as ATCC isolates. Digestion with HaeIII enzyme gave more different bands than with AluI enzyme. It was thought that isolates of E. tarda from turtle and from ATCC were originated from human.