Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Growth And Development Of Protocorm Like Bodies Hybrid Dendrobium Orchids On Ms Medium With Cytokinin And Auxin Combination

The aim of this research was to find out the proper growth regulators for orchid Dendrobium hybrid growth crossed Anching Lubay with Bandung White. The experimental was carried out at Tissue Culture Laboratory of Seed Technology, Faculty Agriculture, Padjadjaran University from middly of August 2006 until middly of December 2006. Completely Randomized Design with eigthteen combination treatments and replicated twice was used in this experiment. The treatments were combination NAA a1,3,5 mg L-1 with BAP 2,4,6 mg L-1 and Thidiazuron 0.02, 0.04, 0.06 mg L-1. Monitoring has been done in a month. Result of experiment showed that number of plb, number of shoots, number of leaves, number of roots, height of shoots, length of leaf and length of root were affected by combination growth regulators. The best result for number of plb was achieved by combination NAA 3 mg L-1 with thidiazuron at 0.06 mg L-1. Combination NAA 3 mg L-1 with thidiazuron 0.04 mg L-1 result the best number of shoots and number of leaves. The best number of root and length of root was achieved by combination NAA 3 mg L-1 and BAP 2 mg L-1. Combination NAA 1 mg L-1 with BAP 4 mg L-1 gave the best length of leaf and height of shoots. The conclusion, combination NAA with TDZ give the best result for number of plb, number of shoots and number of leaves, combination NAA with BAP gave the best result for the number of root and length of root.