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Deteksi Dna Wssv (White Spot Syndrome Virus) Pada Udang Windu Dengan Nested Pcr – Wssv (White Spot Syndrome Virus) Dna Detection In Penaeus Monodon With Nested Pcr

Deteksi Dna Wssv (White Spot Syndrome Virus) Pada Udang Windu Dengan Nested Pcr – Wssv (White Spot Syndrome Virus) Dna Detection In Penaeus Monodon With Nested Pcr
Ibnu Dwi Buwono
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Indonesia
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Metode Nested PCR lebih sensitif mendeteksi white spot syndrome virus (WSSV) dibandingkan dengan one step PCR pada awal infeksi pada udang windu stadia PL30, PL40 dan umur 2 bulan pemeliharaan di tambak. Fragmen DNA WSSV sebagai produk amplifikasi Nested PCR yang berukuran 296 bp diperoleh dari sampel windu PL30, PL40, umur 2 bulan dan udang jembret (vektor virus).

Sampel windu PL30, PL40, umur 2 bulan dan udang jembret asal Karangsong dan Balongan terinfeksi WSSV (296 bp) dengan tingkatan ringan.

Nested PCR methods high sensitive WSSV detected compared with one step PCR on early infection in PL30, PL40 stadia, two months age of Penaeus monodon at brackishwater pond. WSSV DNA fragment as Nested PCR amplification product were sized 296 bp obtained from PL30, PL40 and two months age of Penaeus monodon and Metapenaeus spp. (virus vector) samples.

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