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The Effect Of Tulsi Leaf (Ocimum Sanctum L) Infusion Per Oral On Leydig Cell Count And Sperm Morphologyin Male Mice (Mus Musculus)

The Effect Of Tulsi Leaf (Ocimum Sanctum L) Infusion Per Oral On Leydig Cell Count And Sperm Morphologyin Male Mice (Mus Musculus)
Reni Farenia, Mahadevan Rajasaren, Hanom Husni Syam
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A study have been conducted to find out the effect of the infusion of the Tulsi Leaf (Ocimum Sanctum L) in decreasing the Leydig cell count and effect on sperm morphology of the male mice. The purpose of this research was to find out the effect of the infusion of Tulsi Leaf with 10%, 20% and 30% concentration and to identify which concentration with the highest effect in decreasing the Leydig cell count and which concentration have the highest effect in decreasing the amount of normal sperm morphology of the male mice.
The research is a Prospective Experimental Research, uses treatment with Tulsi Leaf (Ocimum Sanctum L) infusion for 35 days with different concentration of 10%, 20% and 30%,0.5ml/male mice/day. To see whether there is any difference between groups, analysis method which were used is Oneway ANOVA method and PostHoc Test Multiple Comparison method Tukey HSD (ANOVA).

The result of this experiment shows that there is a decrease in the amount of Leydig cell count in male mice of all the treatment group significantly, 5 cells in 10% group, 4 cells in 20% group and 3 cells in 30% concentration group respectively compared with the control group which has a total of 7 cells. The infusion of Tulsi Leaf to the male mice can decrease the amount of normal sperm morphology significantly which for 10% concentration group is 42.4%, 20% concentration group is 37.6% and 30% concentration group is 32.6% respectively compared with the control group which is 58.2%. Group 30% concentration gave the highest effect for decreasing Leydig cell count and decreasing normal sperm morphology of the male mice.

For the conclusion the Tulsi Leaf (Ocimum Sanctum L) infusion which has a positive effect in reducing the Leydig cell count and normal sperm morphology into subfertile stage thus establishes its effect in decreasing male fertility.

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