Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Toxycity Of Extract And Fractions Of Puspa Bark (Schima Wallichii Korth) To Artemia Salina Leach

Puspa (Schima wallichii Korth.) is a medicinal plant which potential to be doveloped as anticancer. Previously research reported that puspa leaves have a cytotoxic activity. In this present works, a toxicity test to extract and fractions of puspa bark was carried out by Brine Shrimp Lethality Test (BSLT). Extraction was done by maceration using ethanol 70%. The concentrated extract was dissolved in water and then fractionated by Liquid-Liquid Extraction (LLE) using n-hexane and ethyl acetate respectively. The result of toxicity test of ethanol extract and fractions of LLE indicated that all sample had a toxic effect to A. salina (LC50 < 1000 ppm). The highest toxycal fraction, that was ethyl acetate fraction (LC50 = 24,70 ppm), then was further separated by classical column chromatoghraphy (CC). The group of fractions II from CC process, showed the highest toxycal activity (LC50 = 11,40 ppm). Preliminary identification of active compound by phytochemical screening and TLC with several spray reagent, predicted that it was terpenoid or polyphenol, however the identity of active compound was still unknown.