Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Anticancer Activity Of Chitosan From Local Chitin Waste Of Fishery Products In Vitro

The aim of the research were to produce local chitin waste to bioactive compound of chitosan enzymatically to applied in functional drink instant. The activity of anticancer this product were determined as in vitro assay. The chitin waste made from crab shells as by product of canning crabs meat industry in Cirebon. Production of chitosan enzimatically used chitin deacetylase enzyme which produced by Bacillus papandayan isolated from Kamojang Creater, West Java, Indonesia. The result of this research was technology of process and chitosan product which degraded enzimatically. Product was chitosan-tea drink which ready to solute in water. Chitosan-tea drink instant performance were white-brown flour mixed with dry chitosan gel (1.5 cm x 1.5 cm), soft surface, brown clear color, and smooth. Formulation of chitosan-tea drink was weight 23.7 g perpack/once consumption (sorbitol 22.5 gram, green tea extract 0.375 gram, chitosan 0.8 gram). Chemically, chitosan-tea drink instant have water content 0.22% w/w, ash content 0.11% w/w, protein content 0.03% w/w, lipid content 0.002% w/w, calory 58.5 kkal which suitable with SNI 01-3722-1995 standard. The product is then tested for toxicity in vitro using AH 109 cancer cells. The test showed that chitosan is cytotoxic to cancer cells AH109 with a value of KI50 (tg / mL) equal to 189.00 for exposure for 8 hours and the value of 1.20 for 24 hours.