Pustaka Ilmiah Universitas Padjadjaran

Abstrak

HYDROLYSIS OF SAGO PITH POWDER (METROXYLON SAGO ROTTB.) IN ENZYMATIC AND FERMENTATION OF HYDROLYZATE BY PICHIA STIPITIS CBS 5773, SACCHAROMYCES CEREVISIAE D1/P3GI, AND ZYMOMONAS MOBILIS FNCC 0056 TO ETHANOL

Sago (Metroxylon sago Rottb) is an abundant source of biomass and have a high starch content, which can be used as a source of renewable energy (bioethanol). The purpose of this study is to obtain the best microorganisms to ferment sugars of the enzymatic hydrolysis yield of sago pith into bioethanol. The research method is experimental in the laboratory consisting of starch hydrolysis and fermentation of sugar pith of sago pith of sago starch hydrolyzate by Pichia stipitis CBS 5773, Saccharomyces cerevisiae D1/P3GI, and Zymomonas mobilis FNCC 0056 in single culture. Pith of sago flour used in this study contained 77.5% starch, 4.86% hemicellulose, cellulose 4.63%, 3.07% lignin and 10.12% water. Through gelatinization and enzymatic hydrolysis of sugar, the sugar concentrations obtained in this study was 47.95% and 61.66% DE. Hydrolysates were used containing 10% sugar, fermented ethanol showed that ethanol production by Pichia stipitis of 2.45% without pH adjustment, 2.19% with a pH adjustment treatment, the concentration of ethanol by Saccharomyces cerevisiae at 3.65% without adjusting the pH, 2.17% with a pH adjustment, and the concentration of ethanol by Zymomonas mobilis at 1.89% for those without pH adjustment and 0.37% with a pH adjustment.