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HYDROLYSIS OF SAGO (METROXYLON SAGO ROTTB.) PITH POWDER BY SULFURIC ACID AND ENZYME AND FERMENTATION OF ITS HYDROLYZATE BY PICHIA STIPITIS CBS 5773, SACCHAROMYCES CEREVISIAE D1/P3GI, AND ZYMOMONAS MOBILIS FNCC 0056 INTO BIOETHANOL

HYDROLYSIS OF SAGO (METROXYLON SAGO ROTTB.) PITH POWDER BY SULFURIC ACID AND ENZYME AND FERMENTATION OF ITS HYDROLYZATE BY PICHIA STIPITIS CBS 5773, SACCHAROMYCES CEREVISIAE D1/P3GI, AND ZYMOMONAS MOBILIS FNCC 0056 INTO BIOETHANOL
Ratu SAFITRI, Bambang MARWOTO, Fenny FIRSTIANTY, Jetty NURHAJATI
Scientific Papers, Animal Science
Inggris
Ratu SAFITRI, Bambang MARWOTO, Fenny FIRSTIANTY, Jetty NURHAJATI , Scientific Papers, Animal Science
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The purpose of this study was to determine hydolysis efficiency of sago pith powder and fermentation efficiency by P. Stipitis CBS 5773, S. cerevisiae D1/P3GI, and Z. mobilis FNCC 0056.. This research was experimentally and consist of hydrolisis process sago pith powder and fermentation of its hydrolyzate. Sago pith powder has content 77,5% starch, 4,63% cellulose, 4,86% hemicelluose, lignin 3,07%, dan water 10,12%. In gelatination process in temperature at 120°C, the yield of sugar concentration 0,62% and dextrose equivalent (DE) 0,89% obtained from the size of 100 mesh. Sago gelatine hydolized by sulfuric acid acid, -amylase, hemicellulase, cellulase, dan amyloglukosidase produce sugar concentration 53,28% and DE 68,52%. At fermentation process in sugar concentration 5% with content of glucose 4.17 %, fermentation by Pichia stipitis, Saccharomyces cerevisiae, and Zymomonas mobilis provide ethanol fermentation efficiency 23,5%, 37,65%, dan 32,13% respectively. At fermentation process with sugar concentration 10% with content of glucose 8,28 %, fermentation by Pichia stipitis, Saccharomyces cerevisiae, and Zymomonas mobilis yielding ethanol fermentation efficiency 9,30%, 50,85% and 20,65% respectively.

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